Glucose Tolerance Tests Accuracy In Diagnosing Diabetes

Glucose Tolerance Tests Accuracy In Diagnosing DiabetesAccording to the World Health Organization (WHO), to a greater extent than 220 trillion hatful worldwide have diabetes. An estimated 1.1 million people died from diabetes in 2005, and almost half of diabetic deaths occurred in people under the age of 70 years of age. WHO projects that the number of diabetic deaths will join on to 366 million by the year 2030 (8).Diabetes Mellitus Type 2 is a prevalent disorder that causes one to have high blood scrape, or hyperglycemia. This hyperglycemia washbasin be the result from one or a combination of 1) decrease production of insulin from beta cells of the pancreas 2) increase sugar production from the liver 3) decrease sugar uptake by cells secondary to insulin receptors. Symptoms of DMII are excess urination, excess thirst, dizziness, blurred vision, sweating, and fatigue. Patients presenting with these symptoms should be screened by a finger stick, where a blood sample is taken fr om a quick prick of the finger, to be tested for hyperglycemia. Normal blood sugar should range from 70-100mg. If one has a fasting sugar of 126mg or an later on eating sugar take aim 200mg, then an oral glucose tolerance test (OGTT) should be performed. During an OGTT, a forbearing consumes a 150-200g carbohydrate diet for three age and fasts from midnight prior to test date. The morning of test, the affected role consumes 75g sugar mixed with 300ml of water within a 5 minute period. The patients blood sugar aim is be measured at baseline, and then again at 120 minutes. A diagnosis of DMII is made if the baseline level is 126 mg and the 120 minute level is 200mg. These guidelines are set by the American Diabetic Association (ADA) and the World Health Organization (WHO) (1,8).A nonher option for obtaining a blood sugar level is measuring the percent of glycosylated red blood cells, or the percent of sugar attached to a RBC. RBCs live for approximately 90 days in the servicem an torso. By measuring this percentile one can observe the patients blood sugar level over the previous 3 months and not just at the moment an OGTT is performed. Today, HbA1c is a main tool for adjacent metabolic control in persons with diabetes(5). A HbA1c 6.0 percent should permit a diagnosis of DMII, but is not at this sentence a definite symptomatic tool.Diabetes can cause complications of multiple organ systems. WHO defines consequences of diabetes as followsDiabetes increases the risk of heart disease and stroke. 50% of people with diabetes die of cardiovascular disease (primarily heart disease and stroke).Combined with reduced blood flow, neuropathy in the feet increases the chance of foot ulcers and tear downtual limb amputation.Diabetic retinopathy is an of import cause of blindness, and occurs as a result of long-term accumulated damage to the small blood vessels in the retina. After 15 years of diabetes, approximately 2% of people become blind, and about 10% develop severe visual impairment.Diabetes is among the leading causes of kidney failure. 10-20% of people with diabetes die of kidney failure.Diabetic neuropathy is damage to the nerves as a result of diabetes, and affects up to 50% of people with diabetes. Although many contrasting problems can occur as a result of diabetic neuropathy, common symptoms are tingling, pain, numbness, or weakness in the feet and hands.The overall risk of dying among people with diabetes is at least double the risk of their peers without diabetes (8).Previous studies have showed that better control of germ plasm glucose levels reduced the risk of developing long-term complications pertaining to diabetes (4). A higher HbA1c correlates well with the likelihood of developing chronic complications such as the ones listed above.This contract is designed to seek if a HbA1c be utilize to diagnose diabetes. Observations suggest that a reliable measure of chronic glycemic levels such as HbA1c, which captures the de gree of glucose exposure over time and which is related more intimately to the risk of complications than single or episodic measures of glucose levels, may serve as a better biochemical marker of diabetes and should be considered a diagnostic tool (2). As for the current g experient standard for diagnosing diabetes, the oral glucose tolerance test (OGTT) has its limitations (2). These include high interindividual variability, low duplicability equated to FPG, poor compliance with the conditions needed to perform the test correctly, and is cumbersome and time-consuming for medical staff and patients (4). Due to these factors one may ask, Is a HbA1c or an OGTT more accurate at diagnosing new onset diabetes mellitus type 2 in a patient presenting with hyperglycemia? By exploring this question and attending it from an render-based approach, the answer may help clinicians advance to an easier and less time consuming way to diagnose diabetes mellitus type II.CLINICAL CASEA 57 year old African American male presented to the outpatient office with symptoms of dizziness, blurred vision, polydipsia, and polyuria. He has a significant annals of hypertension and hyperlipidemia. The patient was unclear when his symptoms started. Upon evaluation in the office, the patient was noted to have a marked glucose elevation of 420. An in-house HbA1c was also noted at 13.0. Upon further questioning, the patient has not been winning any medications for diabetes, and is currently taking Lisinopril and Zocor for his other medical conditions. Due to the presenting symptoms and lab results, the patient was admitted to the hospital for hyperosmolar nonketotic hyperglycemic state.METHODSA PubMed search was performed by using the Clinical queries and diagnosisfilters. The monetary comfort A1c AND diagnosis AND diabetes and glycosylated hemoglobinAND diagnosis AND diabetes were used to search the site for relating articles. With thesesearch terms, a total of 176 hits revealed articl es pertaining to the requested information.Articles that met all inclusion criteria for the research were evaluated and assigned atype/level of evidence.In order to be included in this evidence-based subject area, articles had to meet the following inclusion criteriaArticles must be cohort studies.Studies must not be 6 years old.Articles must have participants with impaired glucose levels or symptoms of impaired glucose.Studies must include evidence of OGTT or FPG and HbA1c.Studies must have a significant number of participants to produce a significant result (n 375).Any articles that did not specifically relate to diagnosing DMII with a HbA1c were excluded. Articles that were not cohort studies, were erstwhile(a) than six years, did not have participants with impaired glucose, or did not have a significant amount of participants were excluded. Certain articles that appeared in the PubMed search were strictly fall over articles. These papers were reviewed, and if applicable, ma y be used to provided supporting factors about pathophysiology/ epidemiology of diabetes type II and its diagnostic criteria. Articles that met all inclusion criteria were evaluated and assigned a level of evidence using the Oxford Centre for show up-based Medicine Levels of Evidence worksheet.RESULTS get hold of 1 Diagnosing Type 2 Diabetes Mellitus in Primary Care, Fasting Plasma Glucose and Glycosylated Hemoglobin Do the Job debate soma This try was performed at the Raval Sud Primary Care Center in Barcelona, Spain and was begun in 1992. The purpose of this training was to determine the inclemency of glycosylated hemoglobin values as a method to diagnose type 2 diabetes mellitus in a population at risk seen in primary care. quad hundred fifty four subjects were selected to participate in the study. The population served by the Raval Sud Center is characterized by it low evonomic level, high rate of immigration, and high rate of unwholesomeness and mortality for certain dis eases and disorders. Inclusion criteria for eligible participants had at least on e of the risk factors for developing DMII expound in the ADA guidelines. These included family history of DMII, personal history of carbohydrate intolerance or gestational diabletes, prolonged use of a drug able to raise glucose levels, obesity with a body mass index 30, hypertension, HDL-cholesterol levels 250 mg/dL. Persons who did not wish to take part in the study were excluded. For the purpose of this particular study, data was recorded from the time the patient was included in the Raval Sud Care Center. The study then used a cross-sectional analytical design to validate a diagnostic test. (4)Study yield Subjects were interviewed and variables were recorded for each participant. These included sociodemographic characteristics such as age and sex, clinical characteristics such as BMI and blood pressure, and laboratory values including fasting plasma glucose in a venous blood sample, oral glucos e tolerance test after a 75g glucose overload, and a HbA1c measured by high pressure liquid chromatography. To standardise the results for the HbA1c, the absolute values were recalculated in terms of the number of standard deviations above the mean. FPG and OGTT values were based on the WHO criteria as having normal, impaired, or DMII glucose levels. (4)Study Results The distribution of demographic characteristics and laboratory findings are shown in Table 1. The study found that plasma glucose levels were significantly overthrow in normal subjects than in subjects with abnormal glucose levels (IFG or OGTT) and even lower in subjects with abnormal glucose levels than in patients with diabetes (P 5.94% (mean, +3SD), the diagnosis of DMII is reliable and accurate in 93% of the cases. Table 4 shows the diagnostic validity of a unite strategy of FPG and HbA1c values patients were considered to have DMII when FPG 125 mg/dL, or when FPG 110 mg/dL and HbA1c was greater than the shortcut value. Maximal efficacy (93% GV) was found for HbA1c 5.94% (x +3SD), with a esthesia of 92.2% and a specificity of 95.1%. (4)Study Critique It has been confirmed that the relationship between circulating glucose values and the onset of chronic complications exists. Thus, it is logical for the diagnosis of DMII to be based on glucose values. One of the main problems in this particular study was to define and establish a cutoff request for this continuous quantitative variable. This study analyzed different cutoff points for the whole sample of patients at risk for DMII. When HbA1c values 5.51% (x +2SD), were used for the cutoff point for diagnosis of DMII, the sensitivity (76%) and specificity (85%) were acceptable. However, when a higher cutoff point was used, specificity increased, but only at the expense of reduced sensitivity. Due to this situation, the study designed a strategy for diagnosis based on the FPG values and the validity of HbA1c. (4) Level of Evidence 1cStudy 2 Comparison of A1c and Fasting Glucose Criteria to Diagnose Diabetes Among U.S. AdultsStudy Design This study included participants from the 1999-2006 National Health and Nutrition interrogative Survey. Participants included 6,890 adults (20 years of age), without a self-reported history of diabetes. The subjects attended a morning examination, fasted for 9 hours at the time of their blood collection, and had valid plasma glucose and HbA1c values taken. Participants were categorize into one of the four groups by presence or absence of fasting plasma glucose 126 mg/dL and HbA1c 6.5%. The distribution of the population into these groupings was determined and the K statistic value was calculated. Also, the distribution of U.S. adults by fasting glucose and different HbA1c cutoff points (6.0-6.7%) were calculated. The objective for this study was to compare A1c and fasting glucose for the diagnosis of diabetes among U.S. adults. (6)Study Conduct Data was collected done questionnair es (demographics, medical history), a physical examination (blood pressure, BMI, and waist circumference), and blood collection (lipids, plasma glucose, HbA1c). The plasma glucose was measured by using a modified hexokinase enzymatic method and the HbA1c using a high-performance liquid chromatography. (6)Study Results This study concludes that an HbA1c of 6.5%, along with a FPG 125 mg/dL demonstrates reasonable agreement for diagnosing diabetes. 1.8% of the participants were classify as having diabetes with a HbA1c 6.5% and a fasting glucose 126 mg/dL. Among participants with a HbA1c 125 mg/dL, 45% had an A1c value 6.0% but less than 6.5%. According to A1c guidelines, this value poses an high-minded risk for diabetes. Table A1 shows a distribution of adults by fasting glucose and different HbA1c cutoff points. From this table, the lower the HbA1c cutoff points results in higher sensitivity and lower specificity. (6)Study Critique In this study, certain participants had discord ant results such as a HbA1c 6.5% and a fasting glucose of Study 3 A1c and Diabetes Diagnosis The Rancho Bernardo StudyStudy Design The Rancho Bernardo Study included 2, 107 participants without known DMII, who had an OGTT and a HbA1c between 1984 and 1987. This cross-sectional study of community dwelling adults was provided written informed consent and laboratory data was performed. (3)Study Conduct HbA1c was measured with high performance liquid chromatography using an automated analyzer. Ophthalmologic evaluation was also performed on the subjects. This was done by using nonmydriatic retinal photography. sensitivity and specificity of HbA1c cutoff points for DMII were calculated, along with K coefficients which were used to test for agreement between A1c values and diabetes status. The objective for this study was to examine the sensitivity and specificity of HbA1c as a diagnostic test for DMII in older adults. (3)Study Results For this study the HbA1c cutoff value was 6.5%. This value had a sensitivity of 44% and a specificity of 79%. A lower A1c cutoff point of 6.15% yielded the highest sensitivity at 63% but a lower specificity at 60%. If one were to use this cutoff value, it would miss tercet of those with DMII by the American Diabetes Association guidelines. It would also misclassify one-third of those without DMII. Using the HbA1c value of 6.5% as the cutoff point, the agreement with DMII diagnosis was low (K coefficient was 0.119). In order to compare A1c and ADA criteria with DMII complications, the study looked at participants with some degree of retinopathy. Of the participants who had retinopathy, 40% had and A1c 6.5% and none had DMII by ADA criteria. This study concluded that the limited sensitivity of the A1c value cutoff may result in missed or delayed diagnosis of DMII, whereas the use of current OGTT criteria will fail to identify a high parity of individuals with high A1c values, which correlate with long term complications of DMII. (3) Study Critique This study was performed on a much older population than the other studies examined in this paper. It has its benefits and disadvantages for canvass a population in which there mean age was 69.4. The advantage is that the U.S. elderly population has the superlative current burden and is expected to have the greatest increase in the prevalence of DMII. On the other hand, the disadvantage to having such an older subject population is that it limited the HbA1c cutoff values to that particular population. In a previous critique of an article one of the concerns was the fact that there are different aspects of glucose metabolism. It would have been supportive if the article addressed the age of their participants and compared them with the study results. (3) Level of Evidence 1cStudy 4 Diagnostic value of glycated haemoglobin (HbA1c) for the early detection of diabetes in high-risk subjectsStudy Design This study was performed by collecting data from the Bundang CHA Gene ral Hospital database. A total of 392 subjects who had an abnormal random plasma glucose, a history of gestational diabetes mellitus, a macrosomic baby, or a severe obesity were selected to participate in the study. Exclusion criteria included a previous history of diabetes of other endocrinopathies, pregnancy, abnormal liver or renal exit tests, a history of major surgery, severe illness, blood transfusion within the previous 6 months, and weight loss 3kg during the past three months. After an nightlong fasting, blood samples were drawn from all participating subjects to include FPG and HbA1c values. (7)Study Conduct Glucose concentrations were measured using the glucose oxidase method on a autoanalyzer. The HbA1c values were measured by the high-performance liquid chromatography method. All statistical analysis was performed and the best predictive cutoff values for FPG and A1c for detecting patients with new diabetes were identified using the optimum sensitivity/specificity va lues determined by the receiver operating characteristic curve. (7)Study Results Figure 1 shows the ROC plot representing the sensitivity and specificity for the HbA1c and the FPG in detecting undiagnosed DMII. From this study, the optimal cutoff value for HbA1c was 6.1% and for FPG was 6.1 mmol/l. The sensitivity/specificity for the HbA1c cutoff value was 81.8% and 84.9% respectively. Table 1 shows the results from the combination of using FPG and HbA1c. This study demonstrated that HbA1c was real useful to screen for diabetes in high-risk patients and the combined use of HbA1c and FPG made up for the lack of sensitivity in FPG alone. (7)Study Critique This studys subjects were only Korean, therefore making the population very ethnically limited. It would have been beneficial to have seen the population more diverse and to notice the change in results. Also, the study stated that an OGTT was performed, yet a tab status of resound testing was not recorded. This would have been be neficial to have in order to compare results to the FPG and HbA1c values obtained for cutoff for diagnosing DMII. (7) Level of Evidence 1cDISCUSSIONThe purpose if this study was to assess if a HbA1c was sufficient enough to make a unknown diagnosis of diabetes mellitus type 2. From these studies one can compile that a HbA1c is adequate for making a new diagnosis for DMII. The following chart compares the specificity and sensitivity of each HbA1c from each study critiqued in this study. Also, each study uses a different HbA1c cutoff that they gathered from their cohort or cross-sectional study which is also included in the chart below.StudySensitivitySpecificityHbA1c used for DiagnosisDiagnosing Type 2 Diabetes Mellitus in Primary Care, Fasting Plasma Glucose and Glycosylated Hemoglobin Do the Job63.3%93.4%5.94%Comparison of A1c and Fasting Glucose Criteria to Diagnose Diabetes Among U.S. Adults72.5%96.5% 6.0%A1c and Diabetes Diagnosis The Rancho Bernardo Study44%79%6.5%Diagnostic v alue of glycated haemoglobin (HbA1c) for the early detection of diabetes in high-risk subjects81.8%84.9%6.1%Study 1 discussed the option of performing a combination of HbA1c and a FPG test. This exhibited to be most the most poignant result with a specificity/sensitivity of 92.2 and 95.1, respectively. In study 2, it also agreed that a HbA1c and a FPG level provided the most assured diagnosis for DMII. However, this study had the most discordant results and was probably cod to the fact of its subject population. It stated that the results may have been due to the fact that assessment of different aspects of glucose metabolism was present (6). Study 3 was performed on a much older population, and focused on the importance of following HbA1c levels to help prevent long term complications of DMII. However, it also stated that a HbA1c would also have a higher sensitivity and specificity if it were performed along with a FPG test. Finally, study 4 agreed on the fact that a HbA1c was ver y sufficient for screening for DMII, and that it provided much support for diagnosing DMII along with a FPG.CONCLUSIONThis study provided that a HbA1c of approximately 6.0% is a great support to help making the diagnosis of DMII along with a FPG 125. Some studies have suggested that a HbA1c of this value is indicative of a diagnosis, however, the studies above advocate that FPG levels should also be obtained to solidify the actually diagnosis of DMII. However, in a recent publication from the JAAP, it states thatan A1c value of 6.5% higher as diagnostic. This value appears to be the level at which a person is at risk for developing the complications of diabetes. A diagnosis should be confirmed with a repeat A1c test, unless clinical symptoms and a glucose level higher than 200 mg/dL are present (5). From this statement one can confer that the patient described above in the clinical case portion of this paper, does indeed warrant the diagnosis of DMII on the basis of a HbA1c of 13. 0%, the presence of clinical symptoms, and the glucose elevation of 420 mg/dL.